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By V. Golemansky, N. Chipev

Dedicated to Gentoo penguins, this quantity discusses the points of genetics, polymorphism, survival, morphology and body structure.

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57 (1) 65 – 71. , P. ZEHTINDJIEV, V. BEZRUKOV, A. SAVOV, Y. YANKOV. 2006. BILL COLORATION OF GENTOO (Pygoscelis papua ellsworthii)PART I: A MARKER FOR GENTOO POPULATION STUDIES. Bulg. Res. 5. , N. BADCOOCK, T. HART, K. SCRIBNER, K. JANSSEN, N. NADEAU. 2004. Conserved Genetic Basis of a Quantitative Plumage Trait Involved in Mate Choice. , A. S. DRANITSINA, M. V. DYBKOV, A. S. VACHEV, S. S. MALIUTA, V. F. GERGIEV. RAPD analysis of gentoo penguins’ populations. Bulg. Res. 5. WILSKE J. (1993) Plasma concentrations of 25-hydroxyvitamin D3 as an indicator of ultraviolet radiatio.

2001). The PCR products in volume of 20 µl were separated on 2% agarose gel and visualized by ethidium bromide staining. RESULTS AND DISCUSSION The yellow spot and its variation During the inspections of Gentoo on Petermann, Wiencke and Livingston Islands we found birds with a clear spot on the upper side of the bill. The spot was variable in size and color: its size ranged from very small (1 – 2 mm) to quite large (20 – 25 mm, spreading over almost all the upper surface of the bill). The color of the spot varied from white (yellowish) to red with yellow, orange and pink intermediate forms.

ZEHTINDJIEV, V. BEZRUKOV, A. SAVOV, Y. YANKOV 40 tubes with K3EDTA or into heparinzed tubes, plasma was separated by centrifugation, erythrocytes were suspended in equal volume of TE buffer (TRIS, EDTA), frozen and stored (under -20 °C) until usage. DNA was extracted using a standard phenol-chloroform method as described by Sambrook et al. (1989). 0 and kept at either 4 or -20 C. Sex determination was performed by PCR, as described by Itoh et al. (2001). The PCR products in volume of 20 µl were separated on 2% agarose gel and visualized by ethidium bromide staining.

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